Research Associate
By | January 5, 2009
BioPhase Solutions specializes in recruiting top talented professionals for San Diego’s Scientific community. We are currently looking for a Research Associate to work for a leading San Diego biotechnology company for a 3 month contract assignment.
Research Associate
DUTIES AND RESPONSIBILITIES:
-Performs experimental procedures from written SOPs
-Assists in the development of new assays
-Contributes to the improvement of existing assays and in the development of new tests
-Assists in the analysis of data utilizing Microsoft Access, Excel and other graphing programs
-Maintains databases as needed
-Performs experiments utilizing molecular biology procedures such as nucleic acid extraction, PCR, RT-PCR, and hybridization assays
-Performs experiments utilizing immunological procedures such as immunostaining, immunoassay and Western Blot techniques
-Assists with assay transfer to the Clinical Laboratory
-Other duties as assigned
EXPERIENCE:
-Strong background in molecular biology
-Assisting the development of genetic typing testing for diagnosing human disease
-Experience in Nucleic acid purification and quantification from human samples
-Experience in assay development specifically in immunoassays such as ELISA
Please visit our website, www.biophaseinc.com, to view additional opportunities in Southern California.
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Sr. QC Chemist-Oligonucleotide Production (San Diego)
By | January 5, 2009
PROVEN Scientific Practice is currently recruiting for a Senior Chemist/QC Specialist to support Oligonucleotide Production.
The successful candidate will contribute to the pursuit of the company’s mission (conception, manufacture and supply of reliable products and services to research institutes and other organizations) by: ensuring the effective operation of all stages of oligonucleotide production maintaining the cost-effectiveness of products and services ensuring customer satisfaction by meeting delivery and quality expectations
Prime Responsibilities
To maintain optimum personal productiveness and conform to objectives with a positive work attitude throughout all stages of oligonucleotide production.
Efficient conduct of procedures in accord with the documentation and with the requirements of the quality management system.
Ensure full collaboration between all sections of oligonucleotide production by the effective communication of all information in relation to orders.
Ensure all technical data and quality control information required by documented procedures is recorded
Draw the attention of the Production Manager to any instances where quality of the technical activities may be called into question
The whole in adherence to: - the companys policy, procedures and the Eurogentec Spirit.
Primary Tasks
Production Activities
Synthesis and purification of highly modified oligonucleotides (such as, but not exclusive to:- RNA, SiRNA, LNA, Beacons..).
Synthesis and purification of oligonucleotides containing new modifications (including existing modifications that have, as yet, never been processed in EGT-NA) and define production processes and prepare SOPs for these.
Test new products for oligonucleotide synthesis and purification (such as, but not exclusive to:- amidites, solid supports for synthesis, purification media, new HPLC column ).
Evaluate existing products from alternative suppliers where appropriate.
Evaluate alternative processes for use in production to improve the production process.
Generate SOPs for new processes that have been approved for production.
Review existing SOPs for synthesis and purification on a 6 month basis
Ensure efficient training of production team members for new processes and techniques.
Verify for accuracy, synthesis details in the production database against those of the Synthesis report sheet and the original order for highly modified oligonucleotides.
Check sequence information and modifications on incoming synthesis report sheets to ensure that the best synthesis, purification and qc methods are used in each case.
Deprotect highly modified oligonucleotides after synthesis.
Prepare MS and OD plates for QC and quantification of crude samples where appropriate.
Quantify the crude samples where appropriate.
Prepare oligonucleotides for purification, and/or desalting.
Remove the deprotection solution from oligonucleotide samples in readiness for purification.
Maintain synthesis records and generate the necessary information in order to re-synthesize oligonucleotides where necessary in order to deliver the product in a timely manner. Inform the production manager and/or the customer service department of any delay occurring in such cases.
Transfer appropriate paperwork and samples to purification or shipping.
Participate in the training of new members of oligonucleotide production team
To development new production methods.
To optimize existing production methods.
QC Activities
To ensure the daily management of QC activities.
Analysis by MALDI
Analysis by ES
Analysis by IE-HPLC
Analysis by RP-HPLC
Analysis by minigel
Functional tests of oligonucleotides used in real time PCR applications
Ensure the transfer of results to production.
To development new QC methods.
To optimize existing QC methods.
To ensure that calibration and performance tests are carried out in accordance with the QMS system.
Inform the customer service department, the production manager and/or the general manager of any delays or problems within QC which will affect the service to the customers.
To ensure that maintenance files are kept up to date for all instruments in production/QC.
Inform the production manager of any production problems that have been identified from the QC analysis of oligonucleotides.
Assist in troubleshooting of failed synthesis.
Generate reports on the QC statistics and present these on the second week of every month during production meetings.
Maintain the data archives (MS and analytical HPLC traces) and samples.
Introduction of new technologies within the oligonucleotide production and ensure validation where appropriate.
Validate all current and future QC methods and processes.
Participate in the validation of all production processes where appropriate.
Assist the health and safety officer where appropriate.
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Research Associate (Sorrento Valley/La Jolla)
By | January 3, 2009
Research Associate BioPhase Solutions
BioPhase Solutions specializes in recruiting top talented professionals for San Diego’s Scientific community. We are currently looking for a Research Associate to work for a leading San Diego biotechnology company.
-Strong background in molecular biology.
-Assisting the development of genetic typing testing for diagnosing human disease.
-Experience in Nucleic acid purification and quantification from human samples.
-Experience in assay development specifically in immunoassays such as ELISA.
ESSENTIAL DUTIES AND RESPONSIBILITIES include the following.
Assists in the development of new assays
Contributes to the improvement of existing assays and in the development of new tests.
Performs experiments utilizing molecular biology procedures such as nucleic acid extraction, PCR, RT-PCR, and hybridization assays
Performs experiments utilizing immunological procedures such as immunostaining, immunoassay and Western Blot techniques
For immediate response please send your resume to the email given in this advertisement. To view additional positions please visit our website www.biophaseinc.com
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research Technician II, lab manager (La Jolla)
By | December 31, 2008
New years special! Position will remain posted until filled.
Research Technician II, Lab Manager, at non-profit Research Center, La Jolla. This position will provide technical support for research in the biology of hematopoietic stem cell. The position requires both cellular and molecular biology skills. Duties will include the isolation of stem cells by flow cytometry, retroviral mediated gene transfer, real time PCR, stem cell and lymphocyte assays. Must be willing to manage and organize laboratory maintenance (ordering, inventories, upkeep of equipment and simple data bases). Organization skills, flexibility, excellent communication skills, and willingness to learn and work with others are essential.
Requires bachelor’s degree in the biological sciences. At least 2 year of hands-on laboratory experience is required. Experience with tissue culture using primary mammalian cells, small animal work, and flow cytometry are a plus. We are looking for a long-term commitment (2 years minimum).
Please email a detailed resume (highlighting experience working in a laboratory) and contact information for 3 references.
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Microbiologist (San Diego)
By | December 17, 2008
MICROBIOLOGISTS
A -TEK, a leading Government Contractor, has exciting career opportunities for results-oriented laboratory professionals in various locations nationwide. We are currently seeking experienced Microbiologist to perform a variety of molecular (particularly PCR) and biological tests to identify the presence of pathogenic microorganisms/agents.
EMPLOYEE BENEFITS:
The individual hired for this position would be eligible to participate in our rich benefits package which includes approximately 90% employer-paid premiums for all employee coverage. The company also supplements the premium payment for spouse and/or dependents coverage under each of the health-care insurance plans only. Most benefits are effective on the first of the month following the date of hire. The following outlines the benefits provided to full-time employees:
Medical Insurance and Prescription Benefits 3 Plan Options available under Anthem BC.BS PPO
Dental Insurance 2 Plan Options under SunLife
Vision Insurance (Vision Service Plan)
Life Insurance (1x annual salary up to $100k max)
Accidental Death & Dismemberment Insurance (1x annual salary up to $100k max)
Long-Term Disability
Short-Term Disability
Professional Development/Tuition Assistance/Professional Associations ($10,500 per year)
401(k) Plan
Premium Only Plan
Paid Leave beginning at 15 days per year
Paid Holidays at 10 days per year
Direct Deposit in up to 3 different accounts
POSITION DESCRIPTION:
Individuals will be responsible for:
Perform a variety of non-research biological science work utilizing microbiological and molecular techniques, including DNA extraction and read-time polymerase chain reaction (PCR) including handling, receipt, and processing of samples, containing potentially high hazard biological agents.
Assuring that incoming specimens are recorded, processed, and triaged appropriately while maintaining chain-of custody.
Reporting test results to appropriate individuals using established procedures.
Applying knowledge of these advanced molecular techniques and microbiological procedures to appropriately interpret and report the findings of the assays.
Performing quality control and quality assurance procedures including, but not limited to routine equipment maintenance and calibration, and documentation of quality control and quality assurance activities as needed. Ensuring that reagents, supplies and equipment are adequately maintained.
Maintaining documentation collection on findings and protocols.
Providing laboratory support for field responses and investigations as directed.
Reporting test results to appropriate individuals using established communication protocols with Local, State and Federal agencies.
Individuals must be available for rotating schedule that provides coverage for a 24hours/7 days a week laboratory operation.
This position routinely works 2 or more weekends per month as directed.
REQUIRED QUALIFICATIONS :
Knowledge of established scientific methods and techniques of microbiology to perform recurring assignment of moderate difficulty (i.e. the methods and techniques are well established, apply to most situations encountered and do not require significant deviation from established methods) or discrete portions of the complex projects.
At least one year laboratory bench experience performing research or diagnostic PCR testing (includes nucleic acid extraction methodology, set up and analyses of real time PCR assays, detection and determination of DNA contamination events).
Minimum one year laboratory bench experience performing research or diagnostic PCR testing (includes nucleic acid extraction methodology, set up and analyses of real time PCR assays, detection and determination of DNA contamination events).
Knowledge of biohazard regulations in the proper and safe handling and transport of materials containing virulent and pathogenic disease agents and toxicants.
Knowledge of chainofcustody regulations and procedures.
Basic knowledge of computer systems methods and applications, including word processing, statistical data management software packages.
Experience in the use of, laboratory instrumentation related to cellular and molecular biology.
Experience maintaining Quality Assurance and Quality Control Documentation and organizational records to ensure adequate reagents and material for 24/7/365 operation.
EDUCATION:
A Bachelors degree from an accredited University in Microbiology, Molecular Biology or related course work in Biological Sciences and at least one year of laboratory bench experience, utilizing polymerase chain reaction (PCR) technology, aseptic techniques and biological assays. Alternatively, equivalent biological laboratory bench experience for at least 4 years is acceptable.
BACKGROUND INVESTIGATION:
Requires passing a law enforcement background check.
May require specific state certifications to work in Public Health facilities, registration with the CDC Select Agent Program, and passing of a Department of Justice background check.
Individual selected will be subject to a background investigation and must meet eligibility requirements for access to sensitive information. The position may require a SECRET clearance.
U.S. Citizenship required.
RESUME SUBMISSION REQUIREMENTS:
1. Submission MUST include your Salary requirement (either as specific amount or acceptable range).
2. Include a reference to job # MCRO - CL1100
3. EMAIL your Resume to LabJobs@a-tek.net or FAX to 703-669-1109.
A-TEK, Inc. is not a recruiting agency
and will not disclose any of your personal information to any outside party.
EOE/M/F/V/D
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Senior Research Associate- Molecular Biology (San Diego)
By | December 3, 2008
Sapphire Energy is a venture capital backed company developing next generation biofuels based on recent advances in metabolic engineering. The companys focus is the commercialization of high performance, low cost, and low carbon footprint that will substitute for conventional gasoline, diesel, and jet fuel.
We are seeking a B.S. or M.S. level Senior Associate position with Molecular Biology, Biochemistry or closely related disciplines to join the Molecular Biology Department.
Responsibilities include high-throughput strategies for cloning, protein expression screening, and downstream purification processing and characterization. The ideal candidate will lead a group and work closely within a team of scientists responsible for providing highly purified proteins to support the HTS and biochemical groups within the pipeline. Preference will be given to applicants with previous experience in high-throughput proteomics initiatives, and with 4-7 years post-baccalaureate research experience in an industrial setting.
Candidates will lead a group primarily for the cloning, screening and expression in algae cells, in E. coli-based cloning and expression or in downstream protein processing and characterization, with some degree of overlap between positions.
Requirements:
4+ years experience in some or all of the following:
Thorough understanding and working knowledge of contemporary high-throughput cloning and purification screening strategies
Plate-based high-throughput cloning procedures using restriction-based and ligation-independent strategies
Plate-based transformation protocols and clone identification using diagnostic PCR
High-throughput preparation of plasmid DNAs suitable for DNA sequencing
Aseptic cell culture techniques
Plate-based generation of E. coli expression cultures and preparation of total and soluble fractions
High-throughput analytical HPLC chromatographic analysis of recombinant proteins
High-throughput mass spectrograph analysis of recombinant proteins
High-throughput analysis of recombinant protein purity using SDS-PAGE and IEF analysis
High-throughput proteolytic fragmentation analysis of recombinant proteins by mass spectrograph and SDS-PAGE
Candidates should possess excellent communication and problem solving skills, be motivated, able to multi-task and lead a team, to work independently if necessary, and to maintain detailed laboratory records. In addition, a track record of successful teamwork in a multidisciplinary environment is highly desired.
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Girl Boutique Key Holder Positions (North County & Plaza Camino Real Mall)
By | December 3, 2008
Key holder positions are available at two Romy Boutique locations. Open availability is a must for this position. Must have strong people skills and enjoy working in retail. At least one year of key holder experience necessary. Fun atmosphere to work in at our North County and Plaza Camino Real locations. There are also monthly chances to win gift certificates and cash prizes at Romy. Come work with an expanding quality clothing company.
If applying to the Plaza Camino Real position send resume to Sonia at mgr.pcr@romystyle.com
If applying to the North County position send resume to Sarena at sarenac@romystyle.com
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Biotech Research Assistant (San Diego, CA)
By | December 1, 2008
Our client is a leading biotech research company seeking additional personelle for their R&D department. This position requires previous lab and/or industry experience.
Job Description:
Under supervision, this person will be preparing media plates, buffer solutions, and glasswashing, and completing lab inventory. Prior experience with PCR, protein purification, DNA extraction, and HPLC is required. Knowledge of Microsoft Office and Excel is a must.
Requirements:
Bachelors of Science with 0-2 years lab/industry experience.
For immediate consideration, please contact Brian Riehle at 619-278-3022 and email your resume and salary history to briehle@aerotek.com
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Research Associate, Genomics at Prognosys Biosciences (La Jolla)
By | November 10, 2008
Job Code: 2008-10-RD
Join a dedicated team of scientists working to develop and apply high-throughput technologies to enable predictive medicine.
Responsibilities:
Participate in the development of genomics and proteomics based assays with applications in human health.
Synthesize and use high-density DNA microarrays.
Participate in innovative research projects funded by the National Institutes of Health (NIH).
Qualifications:
BA or BS degree in Molecular Biology, Biochemistry, or Chemistry.
Experience in basic molecular biology techniques including RNA and DNA purification, PCR, and enzymology. Solid foundation in chemistry.
Relevant work experience is an advantage.
Accuracy and attention to detail are essential.
Excellent organizational, documentation, and communication skills.
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Research Associates - Molecular Biology/Microbiology (Sorrento Valley)
By | October 31, 2008
Available immediately, an industrial position in a research-oriented group making lipid products in microbes using both molecular and physiological approaches.
Qualifications:
BA/BS degree in Molecular Biology, Biochemistry, Microbiology, or similar discipline and 1-3 years experience.
Molecular biology
· Working familiarity with Vector NTI or similar bioinformatics program
for primer design, sequence alignment, BLAST searches, contig assembly
and sequence analysis
· DNA and RNA isolation
· Basic cloning skills, including cloning genes from genomic DNA
· Plasmid construction
· Site-directed Mutagenesis
· PCR, including optimization of primers and reaction conditions
Microbiology
· DNA transformation
· Basic microbiological methods, focus on Yeast a plus
Desirable:
· Experience in culturing in shakeflasks and scaling up to fermenters
· Media design
· Strain construction: strain mutagenesis and designing selection/screens.
Flexibility and adaptability are highly valued.
Located in Sorrento Valley, Cibus LLC is an agricultural biotechnology company. Our website is: www.cibusllc.com.
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