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Research Associate – Cancer Biology (berkeley)

By | July 30, 2007

Lawrence Berkeley National Laboratory (LBNL) is a world leader in science and engineering research, with 11 Nobel Prize recipients over the past 75 years, and 59 present members of the National Academy of Sciences. LBNL conducts unclassified research across a wide range of scientific disciplines and hosts four national user facilities. The Life Sciences Division at LBNL applies expertise in computation, bioinformatics, cutting-edge imaging technologies and structural cell biology to further understand the complexity of biological systems. Learn more at http://www.lbl.gov/lifesciences/.

The Cancer Biology department has an immediate need for a Research Associate to isolate human mammary epithelial cell (HMEC) sub-populations for experiments, contribute to HMEC culture systems development, and perform ongoing assays for biological and molecular properties.

Additional Responsibilities:

• Assess the effects of 2D and 3D culture conditions on parameters of normal HMEC growth, differentiation, and senescence

• Maintain and become familiar with a wide variety of finite and transformed HMEC cultures

• Maintain and perform data entry of records pertaining to HMEC cultures and usage

• Perform common assays involving biological and molecular characterization of HMEC, e.g., flow sorting (FACS), immunohistochemistry and immunofluorescence, western, northern, and southern blotting, and quantitative-RT-PCR amplifications

• Perform analysis for detection of mycoplasm contamination in cell cultures

• Provide protocol details for experiments designed by principal investigator

• Prepare media, buffers, and reagents

• Generate data for investigator’s use in preparation of technical reports and publications

Qualifications:

• B.S. degree in a related field and/or at least 2 years of hands-on experience

• Ability to discern differences between cell cultures subjected to different treatments

• Ability to successfully cultivate HMEC cultures

• Hands-on experience in many of the following molecular biology techniques/assays: gel electrophoresis, western, southern, and northern blotting, immunohistochemistry, immunofluorescence, PCR, RT-PCR, and flow cytometry

• High attention to detail as well as ability to effectively handle multiple priorities

• Familiarity with PC hardware and software such as MS Word and MS Excel

Note: This position is represented by a union for collective bargaining purposes.

Please apply at http://jobs.lbl.gov/LBNLCareers/details.asp?jid=20668&p=1. Submit a single attachment including your résumé and cover letter. Reference “Internet” and “Craigslist” as your source.

LBNL is an AA/EEO employer committed to the development of a safe and diverse workforce. Learn more at www.lbl.gov.

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