Research Laboratory Technician (UNC Chapel Hill Dept of Genetics)
By | March 4, 2010
LABORATORY TECHNICIAN WANTED
In the laboratory of Dr. Daniel Pomp
Dept. Genetics UNC Chapel Hill
Minimum Job Description:
Mouse handling and colony management (experience with wild strains preferred),
tissue collection, DNA and RNA isolation, PCR.
Weekend and evening availability required.
Applicants with mouse brain dissection and sectioning skills encouraged to apply.
Position Available March, 2010
Full Time Temporary Position
The University of North Carolina at Chapel Hill is an Equal Opportunity Employer.
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Scientist II (Morrisville)
By | March 4, 2010
Processes client samples using approved standard operating procedures
Performs routine and non-routine intermediate to high complexity pharmacogenomic assays in a GLP regulated environment including DNA purification and quantitation, gel electrophoresis, real-time PCR, and DNA Sequencing
Perform RNA extraction, cDNA synthesis and total RNA quality analysis from a variety of sources including FFPE tumor samples using approved standard operating procedures
Absolute and/or relative quantification of gene expression levels using real-time PCR methods
Review of gene expression data, gene expression protocols and final reports
Ability to perform quality work in a RNase-free environment
Perform the laboratory portion of the assay development and validation process with minimal supervision including optimization of PCR conditions
Work is consistently high in quality
Assess and prioritize daily activities and communicate/update study directors and the Lab Manager as necessary
Assists in identifying and troubleshooting discrepancies and quality control failures
Assists in the writing, editing, and implementing standard operating procedures, material requirements documents, and other essential documentation
Assists in the development and validation of new assays
Maintains equipment logs, maintenance logs, and calibration logs
Assists in laboratory inventory, makes common reagents and reviews reagent quality control
Requires a minimum of a Bachelors Degree with a concentration in life sciences, Molecular Biology, Biochemistry, Pharmacology, or closely related field. Must be able to multitask and have good time management skills. Must be able to work in a team atmosphere, have experience in aseptic techniques, and have a positive attitude toward work environment.
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Scientist III (Morrisville)
By | March 4, 2010
Essential Duties and Responsibilities:
This position is a bench scientist. Genetic analysis using the Affymetrix arrays (DMET, 3 IVT and whole transcript gene expression arrays). *Excellent skills in experimental design, implementation, troubleshooting, and data analysis. *Experience handling large datasets generated from DNA and/or Microarray analysis. *Performs RNA extraction, total RNA quality analysis and cDNA synthesis from a variety of sources including FFPE tumor samples using approved standard operating procedures. *Perform absolute and/or relative quantification of gene expression levels using real-time PCR methods. *Review of gene expression data, gene expression protocols and final reports. *Performs DNA purification and quantification, gel electrophoresis, and normalization. *Performs routine and non-routine genotyping assays including DNA sequencing, gel based assays and endpoint analysis. *Performs the laboratory portion of the assay design and development and validation process with including optimization of PCR conditions. *Assay design for the detection human genetic variations using bioinformatic software and other appropriate resources. *Perform literature searches in support of the scientific efforts at Gentris. *Develops protocols and methods for evaluating and analyzing various types of genomics data. *Assists in the writing, editing, and implementing standard operating procedures, material requirements documents, and other essential documentation. *Mentoring and training staff in the junior scientists positions. *Communicate ongoing project/study progress and act as the key interface to clients.
Minimum requirements include a Biological Science degree (BS+4, MS+2) with Industry experience, Biotech / Pharmaceutical / Drug Discovery. Candidates must have working experience with molecular biology techniques, and knowledge of advanced molecular biology techniques especially with RNA and microarray chip technology. Must be detail-oriented. Ability to think and work independently.
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Bench Scientist - contract position (RTP, NC )
By | February 22, 2010
Our client in RTP, NC, seeks a Research Pharmacologist/Bench Scientist for a long term contract position. The selected candidate would work in the Department of Pharmacology and Toxicology focused on molecular pharmacology and cell-based assay development. This department’s work is currently focused on generating stable cell lines expressing protein targets of interest to treat drug addiction and CNS disorders. They develop and validate new assays and then run the assays to evaluate novel compounds. The ideal candidate will have a strong desire to use data interpretation skills.
Job Responsibilities:
Generate stable cell lines expressing targets of interest
Create expression and reporter plasmids using standard subcloning and PCR techniques
Perform various cell-based assays
Evaluate interim results in generation of plasmids and cell lines, troubleshoot processes.
Support experimental design including appropriate controls
Assist colleagues in experimental design and troubleshooting
Learn and apply additional techniques as needed
Position/Educational Requirements:
BS/MS degree
Sterile cell culture and molecular biology experience is required
Strong data interpretation
Significant experimental design and troubleshooting skills are required.
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Molecular Biologist (RTP)
By | February 17, 2010
Advanced Liquid Logic Inc. (www.liquid-logic.com ) is a well-funded early-stage company that develops microfluidic lab-on-a-chip devices for a diverse range of applications including medical diagnostics, drug discovery, environmental monitoring and research instrumentation. Advanced Liquid Logic is commercializing digital microfluidics a lab-on-a-chip technology in which nanoliter-sized droplets are programmably manipulated using electrical fields. Digital microfluidics is an emerging and highly interdisciplinary field at the intersection of engineering, biology, medical diagnostics, physics and materials science.
DUTIES AND RESPONSIBILITIES:
Support the research and development of microfluidic devices in life sciences applications including DNA sequencing, sample preparation and PCR
Optimize chemistries and protocols for translation to droplet-based microfluidic format, including especially pyrosequencing and genomic sample preparation protocols
Develop reagent quality testing methods and establish quality control measures for routine use and long-term storage of reagents
Work closely with engineers and technicians to translate bench-format assays to microfluidic format
Assist with the transition of research results to the development of products
Maintain impeccable lab notebooks documenting experimental methods and results; assist with developing and filing intellectual property; assist with writing of grant proposals and reports
QUALIFICATIONS/CHARACTERISTICS:
Ability to work in a highly interdisciplinary environment along-side engineers, biochemists, and technicians; ability to work under minimal supervision
High integrity, insightful, industrious, team player, highly intelligent and most importantly, passionate about their work
Excellent verbal and written communications skills; strong analytical and computer skills for design of experiments and data analysis
EDUCATION AND EXPERIENCE REQUIRED:
PhD in Biochemistry, Molecular Biology or relevant discipline
Strong background in nucleic acid biochemistry
Minimum of three years experience in an academic or industrial laboratory
Pyrosequencing, next generation DNA sequencing or microfluidics experience a plus
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Scientist III (Morrisville)
By | February 15, 2010
Essential Duties and Responsibilities:
This position is a bench scientist. Genetic analysis using the Affymetrix arrays (DMET, 3 IVT and whole transcript gene expression arrays). *Excellent skills in experimental design, implementation, troubleshooting, and data analysis. *Experience handling large datasets generated from DNA and/or Microarray analysis. *Performs RNA extraction, total RNA quality analysis and cDNA synthesis from a variety of sources including FFPE tumor samples using approved standard operating procedures. *Perform absolute and/or relative quantification of gene expression levels using real-time PCR methods. *Review of gene expression data, gene expression protocols and final reports. *Performs DNA purification and quantification, gel electrophoresis, and normalization. *Performs routine and non-routine genotyping assays including DNA sequencing, gel based assays and endpoint analysis. *Performs the laboratory portion of the assay design and development and validation process with including optimization of PCR conditions. *Assay design for the detection human genetic variations using bioinformatic software and other appropriate resources. *Perform literature searches in support of the scientific efforts at Gentris. *Develops protocols and methods for evaluating and analyzing various types of genomics data. *Assists in the writing, editing, and implementing standard operating procedures, material requirements documents, and other essential documentation. *Mentoring and training staff in the junior scientists positions. *Communicate ongoing project/study progress and act as the key interface to clients.
Minimum requirements include a Biological Science degree (BS+4, MS+2) with Industry experience, Biotech / Pharmaceutical / Drug Discovery. Candidates must have working experience with molecular biology techniques, and knowledge of advanced molecular biology techniques especially with RNA and microarray chip technology. Must be detail-oriented. Ability to think and work independently. Salary range $40-50K, commensurate with experience
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Research Technician (RTP)
By | February 14, 2010
Company overview:
Nanosyn is an integrated chemical and biological services contract research organization providing innovative solutions in the areas of chemical libraries synthesis, medicinal chemistry, high-throughput bioactivity profiling and assay development services.
Job Description:
This position will support the operation of biology R&D group by performing basic molecular biology laboratory techniques including: mammalian cell culture, DNA cloning, RNA and DNA isolation, protein purification, PCR, SDS-PAGE electrophoresis, western blotting, ELISA etc.
Duties
Perform day-to-day supervised molecular and cell biology experiments
Maintain sufficient inventory of material, supplies and equipment for performance of duties
Clean and maintain laboratory glassware and equipment.
Prepare common solutions/buffers
Qualifications:
Successful applicant should have:
B.S./M.S. in biological sciences
Background in biochemistry, molecular and cell biology is preferred
Experience with protein expression and purification is a plus.
At least 2 years of relevant experience in the molecular biology lab.
Contact information:
Please send you resume to:
Jowita Mikolajczyk at: jmikolajczyk@nanosyn.com
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Accredited DME Co. Seeks PCR’s (Triangle -NC)
By | February 11, 2010
We are a North Carolina Durable Medical Equipment Supplier of home medical equipment and diabetic supplies. Do to the medical business sector’s exploding growth, we are in need of caring, empathic and professional sales representatives to demonstrate and educate the Senior Citizen & Disabled markets on how they can benefit using our equipment.
Duties include, but not limited to educating prospective patients on how they can acquire needed equipment & supplies with little to no out of pocket cost to them. Demonstrating equipment, assist prospective patients with filing claims, offer exceptional customer service.
If you feel you can deliver energy, compassion, relationship building skills then reply to randy@tarheeldiabetic.com with your resume attached. I will choose the resumes that fit best, call or email you a invitation for interview and tour our facilities. You will have to travel to our headquarters in St. Paul’s, NC for interview. NO TELEPHONE INTERVIEWS WILL BE GIVEN. Interviewing Wednesday02-17-10. Submit your resume today for consideration.
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Cytogenetics Team Leader
By | February 10, 2010
The Cytogenetics department is seeking a Laboratory Services Team Leader position to join their blood group. This position will have hours from 8am - 5pm Sunday - Thursday.
Employee must have 7 years of Cytogenetic experience and be ASCP certified. The team lead will have responsibilities in the routine blood group, including but not limited to: availability as a resource for less experienced cytogenetics technologist; specimen tracking; analysis of unusual/difficult cases; processing review; and other tasks as needed. A familiarity with CAP and New York regulations is a plus.
The Cytogenetics department is housed in the Center for Molecular Biology and Pathology (CMBP). CMBP is celebrating 20 years of service in the RTP area, pioneering polymerase chain reaction (PCR) and standing as a leader in diagnostic genetics testing.
If interested, please apply online at http://track.jobviper.com/ViewJob.asp?id=659063-1248-3836
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Research Pharmacologist/Bench Scientist (RTP, NC)
By | February 5, 2010
Our client in RTP, NC, seeks a Research Pharmacologist/Bench Scientist for a long term contract position. The selected candidate would work in the Department of Pharmacology and Toxicology focused on molecular pharmacology and cell-based assay development. This department’s work is currently focused on generating stable cell lines expressing protein targets of interest to treat drug addiction and CNS disorders. They develop and validate new assays and then run the assays to evaluate novel compounds. The ideal candidate will have a strong desire to use data interpretation skills.
Job Responsibilities:
Generate stable cell lines expressing targets of interest
Create expression and reporter plasmids using standard subcloning and PCR techniques
Perform various cell-based assays
Evaluate interim results in generation of plasmids and cell lines, troubleshoot processes.
Support experimental design including appropriate controls
Assist colleagues in experimental design and troubleshooting
Learn and apply additional techniques as needed
Position/Educational Requirements:
PhD
Sterile cell culture and molecular biology experience is required
Strong data interpretation
Significant experimental design and troubleshooting skills are required.
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