Scientist III (Morrisville)
By | March 4, 2010
Essential Duties and Responsibilities:
This position is a bench scientist. Genetic analysis using the Affymetrix arrays (DMET, 3 IVT and whole transcript gene expression arrays). *Excellent skills in experimental design, implementation, troubleshooting, and data analysis. *Experience handling large datasets generated from DNA and/or Microarray analysis. *Performs RNA extraction, total RNA quality analysis and cDNA synthesis from a variety of sources including FFPE tumor samples using approved standard operating procedures. *Perform absolute and/or relative quantification of gene expression levels using real-time PCR methods. *Review of gene expression data, gene expression protocols and final reports. *Performs DNA purification and quantification, gel electrophoresis, and normalization. *Performs routine and non-routine genotyping assays including DNA sequencing, gel based assays and endpoint analysis. *Performs the laboratory portion of the assay design and development and validation process with including optimization of PCR conditions. *Assay design for the detection human genetic variations using bioinformatic software and other appropriate resources. *Perform literature searches in support of the scientific efforts at Gentris. *Develops protocols and methods for evaluating and analyzing various types of genomics data. *Assists in the writing, editing, and implementing standard operating procedures, material requirements documents, and other essential documentation. *Mentoring and training staff in the junior scientists positions. *Communicate ongoing project/study progress and act as the key interface to clients.
Minimum requirements include a Biological Science degree (BS+4, MS+2) with Industry experience, Biotech / Pharmaceutical / Drug Discovery. Candidates must have working experience with molecular biology techniques, and knowledge of advanced molecular biology techniques especially with RNA and microarray chip technology. Must be detail-oriented. Ability to think and work independently.
Topics: Raleigh-Durham PCR jobs | No Comments »
Scientist II (Morrisville)
By | March 4, 2010
Processes client samples using approved standard operating procedures
Performs routine and non-routine intermediate to high complexity pharmacogenomic assays in a GLP regulated environment including DNA purification and quantitation, gel electrophoresis, real-time PCR, and DNA Sequencing
Perform RNA extraction, cDNA synthesis and total RNA quality analysis from a variety of sources including FFPE tumor samples using approved standard operating procedures
Absolute and/or relative quantification of gene expression levels using real-time PCR methods
Review of gene expression data, gene expression protocols and final reports
Ability to perform quality work in a RNase-free environment
Perform the laboratory portion of the assay development and validation process with minimal supervision including optimization of PCR conditions
Work is consistently high in quality
Assess and prioritize daily activities and communicate/update study directors and the Lab Manager as necessary
Assists in identifying and troubleshooting discrepancies and quality control failures
Assists in the writing, editing, and implementing standard operating procedures, material requirements documents, and other essential documentation
Assists in the development and validation of new assays
Maintains equipment logs, maintenance logs, and calibration logs
Assists in laboratory inventory, makes common reagents and reviews reagent quality control
Requires a minimum of a Bachelors Degree with a concentration in life sciences, Molecular Biology, Biochemistry, Pharmacology, or closely related field. Must be able to multitask and have good time management skills. Must be able to work in a team atmosphere, have experience in aseptic techniques, and have a positive attitude toward work environment.
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Research Laboratory Technician (UNC Chapel Hill Dept of Genetics)
By | March 4, 2010
LABORATORY TECHNICIAN WANTED
In the laboratory of Dr. Daniel Pomp
Dept. Genetics UNC Chapel Hill
Minimum Job Description:
Mouse handling and colony management (experience with wild strains preferred),
tissue collection, DNA and RNA isolation, PCR.
Weekend and evening availability required.
Applicants with mouse brain dissection and sectioning skills encouraged to apply.
Position Available March, 2010
Full Time Temporary Position
The University of North Carolina at Chapel Hill is an Equal Opportunity Employer.
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Staff Research Associate II (pacific heights)
By | March 4, 2010
The Korn Laboratory at the UCSF Helen Diller Family Comprehensive Cancer Center and the Division of Gastroenterology is seeking a highly motivated, dynamic individual with strong background in cellular and molecular biology to work as a staff research associate with post-doctoral fellows. The candidate will join a highly skilled and collegial team of scientists. The laboratory is dedicated to the development of new cancer treatment strategies based on in-depth understanding and rational targeting of signal transduction pathways.
Responsibilities:
Cell culture maintenance and experimentation
RNA and protein expression analysis (PCR, Western blotting, Luminex assays, FACS analysis)
Immunofluorescence microscopy
Cell viability analysis
Maintenance and ordering of lab supplies
Qualifications:
Graduation from college with a BS or BA degree in biomedical or physical sciences
Experience with basic laboratory techniques, including DNA, RNA, and protein isolation, PCR, Western blot analysis, mammalian cell culture
computer (Macintosh or PC) literacy
facility with scientific English
The application should include current CV as well as three references with contact information.
UCSF is an Equal Opportunity/Affirmative Action Employer. The University undertakes affirmative action to assure equal employment opportunity for underutilized minorities and women, for persons with disabilities, and for covered veterans. All qualified applicants are encouraged to apply, including minorities and women.
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Sr. Research Associate - Temp
By | March 4, 2010
BioPhase Solutions specializes in recruiting top talented professionals for San Diego’s Scientific community. We are currently looking for a Senior Research Associate, Molecular Technology to work for a leading San Diego biotechnology company.
Senior Research Associate
Description:
The successful candidate will utilize current and novel recombinant DNA/molecular biological approaches to clone and express proteins of therapeutic value for the treatment of cancers and metabolic diseases. The candidate will play an active role to expand the company technology platform, develop mammalian production cell lines, improve mammalian, and facilitate protein engineering.
Job Requirements:
* B.S./M.S. in a scientific discipline with at least 5 years of full time laboratory research.
* Experience in molecular biology techniques, such as plasmid construction, site-directed mutagenesis, PCR, DNA sequencing.
* Experience with mammalian cell tissue culture and cell line development.
* Experience with bacterial culture and aseptic techniques.
* Experience with ELISA, PAGE, UV quantification.
* Must be able to work effectively in a fast-paced environment.
For all candidates, excellent computer, communication, organization and collaborative skills are essential. Previous experience in a Biotech or Pharmaceutical environment is preferred.
Please visit www.biophaseinc.com to view additional opportunities throughout San Diego County!
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Associate Scientist (Level II) (Cambridge, MA)
By | March 2, 2010
An Associate Scientist (Level II) will work in the Research Biology Department at the ALS-Therapy Development Institute in Cambridge, MA to support in vivo and in vitro validation of candidate therapeutics in murine models of Amyotrophic lateral sclerosis (ALS). This candidate will be responsible for the following three jobs:
1. Weekly genotyping of transgenic mice using an in-house, automated Q-PCR platform.
2. Support and development of enzyme linked immunosorbent assays (ELISA) for weekly, large-scale pharmacokinetic studies.
3. Development of assays for protein characterization such as immunoblots, immunoprecipitation, and immunohistochemistry.
The candidate will work closely with internal in vitro and in vivo research teams, as well as external vendors, to support ongoing preclinical studies in a mouse model of ALS.
Academic Qualifications and Technical skills:
The candidate must have a BS or MS in biology, biochemistry, or molecular biology and 3+ years of experience in molecular biology techniques including nucleic acid purification, PCR, Q-PCR, ELISA, and immunoblotting. Working knowledge of spreadsheet programs, is also required. Ideally, the candidate will have experience with molecular biology automation.
Strong communication skills and a team orientation are essential.
The Amyotrophic Lateral Sclerosis Therapy Development Institute (ALS-TDI) is a non profit biotech focused on the discovery and development of therapeutics to treat ALS. The institute is a world leader in the pharmacological testing of lead candidates in animal models of ALS and has established expertise in the utilization of genomics and proteomics technologies for drug discovery.
Please submit resume and cover letter.
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Lab technician/lab manager position at UCSF (inner sunset / UCSF)
By | March 2, 2010
The Engel laboratory in the Microbial Pathogenesis and Host Defense program at UCSF is seeking a talented and highly motivated individual with a strong background in Cell biology and Microbiology to serve as our laboratory manager and to provide experimental support for our postdoctoral fellows with their projects. The laboratory is an exciting, collaborative, and dynamic environment that uses cutting edge research techniques to investigate the interactions between bacterial pathogens and host cells with a special emphasis on Pseudomonas and Chlamydia infections
Responsibilities include Lab management (ordering/organizing laboratory supplies, basic maintenance duties), Molecular biology techniques (RNA/DNA/protein isolation, blotting, PCR, cloning), Cell culture maintenance and experiments, Advanced microscopy including indirect immunofluorescence, confocal microscopy, videomicroscopy, Supervision of lab assistant.
Qualifications: Bachelor in Biology or related discipline, with at least 3 years of lab experience; Willingness to make a minimum 2 year commitment to the laboratory; Knowledge of general laboratory techniques that include: PCR; DNA, RNA, and protein isolation, cloning DNA, mammalian cell culture, bacterial culture, sterile technique; Experience in microscopy; Computer proficiency with both Mac and PC platforms; motivated and detail oriented individual who works well in a highly collaborative environment, who readily learns new techniques, who is capable of multi-tasking, and who has strong written, verbal, data recording, and organizational skills.
The application should consist of your CV and the names of 3 references emailed to rluu@medicine.ucsf.edu. Please title your e-mail “Engel lab tech job”. Please direct questions to the same email address.
UCSF is an Equal Opportunity/Affirmative Action Employer. The University undertakes affirmative action to assure equal employment opportunity for underutilized minorities and women, for persons with disabilities, and for covered veterans. All qualified applicants are encouraged to apply, including minorities and women.
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Research Technician II (Sorrento Valley)
By | March 1, 2010
InVivoScribe Technologies, a rapidly growing and profitable biotechnology company, has an immediate opening for a full-time Research Technician II. Candidates must have excellent written and verbal communication skills, a strong work ethic, a high attention to detail, computer literacy, and the ability to work independently following verbal instructions and SOPs.
Primary Duties and Responsibilities include the following:
Carry out experiments including, but not limited to PCR reactions, Q-PCR reactions, agarose/polyacrylamide gel electrophoresis, TOPO cloning, bacterial cell culture,, mammalian cell culture, enzyme assay, and protein purification on an FPLC/HPLC using a variety of purification resins.
Maintain a laboratory notebook that records all aspects of experimental design and results.
Ensure that laboratory reagents are properly stocked and that laboratory bench is clean and clutter-free.
Requirements:
Requires knowledge and skills normally acquired through the successful completion of a BS Degree in a scientific discipline.
Requires over 2 years of laboratory experience in a molecular biology or equivalent combination of education and experience
Ability to consistently meet critical deadlines.
Attention to detail.
Ability to follow Standardized Operating Procedures (SOPs).
The ability to work independently as well as in a team environment.
Excellent communication skills with the ability to train employees.
The ability to manage multiple projects and changing priorities.
Willingness to learn and take on new challenges.
Ability to perform some tasks that are monotonous & physically demanding in lab environment.
Occasional lifting of up to 30 pounds.
Some activities will take place at a desk and on a computer.
Skills, Knowledge & Abilities Preferred, but not Required:
Knowledge of graphics software such as Adobe Photoshop, Illustrator, etc.
Inventory maintenance experience.
Experience with Adobe software.
FAX/email resume to 858-623-8109 or HR1@invivoscribe.com.
Please, no phone calls or walk-ins about this job!
Salary: Commensurate with experience
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Research Technician II (Sorrento Valley)
By | March 1, 2010
InVivoScribe Technologies, a rapidly growing and profitable biotechnology company, has an immediate opening for a full-time Research Technician II. Candidates must have excellent written and verbal communication skills, a strong work ethic, a high attention to detail, computer literacy, and the ability to work independently following verbal instructions and SOPs.
Primary Duties and Responsibilities include the following:
Carry out experiments including, but not limited to PCR reactions, Q-PCR reactions, agarose/polyacrylamide gel electrophoresis, TOPO cloning, bacterial cell culture,, mammalian cell culture, enzyme assay, and protein purification on an FPLC/HPLC using a variety of purification resins.
Maintain a laboratory notebook that records all aspects of experimental design and results.
Ensure that laboratory reagents are properly stocked and that laboratory bench is clean and clutter-free.
Requirements:
Requires knowledge and skills normally acquired through the successful completion of a BS Degree in a scientific discipline.
Requires over 2 years of laboratory experience in a molecular biology or equivalent combination of education and experience
Ability to consistently meet critical deadlines.
Attention to detail.
Ability to follow Standardized Operating Procedures (SOPs).
The ability to work independently as well as in a team environment.
Excellent communication skills with the ability to train employees.
The ability to manage multiple projects and changing priorities.
Willingness to learn and take on new challenges.
Ability to perform some tasks that are monotonous & physically demanding in lab environment.
Occasional lifting of up to 30 pounds.
Some activities will take place at a desk and on a computer.
Skills, Knowledge & Abilities Preferred, but not Required:
Knowledge of graphics software such as Adobe Photoshop, Illustrator, etc.
Inventory maintenance experience.
Experience with Adobe software.
FAX/email resume to 858-623-8109 or HR1@invivoscribe.com.
Please, no phone calls or walk-ins about this job!
Salary: Commensurate with experience
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RESEARCH ASSOCIATE (Medford, MA)
By | February 26, 2010
Hepregen Corporation (200 Boston Ave, Suite 1500, Medford, MA 02155, www.hepregen.com) is a venture-backed startup out of the Massachusetts Institute of Technology focused on commercializing a portfolio of liver tissue engineering technologies. Hepregens mission is to provide innovative bioengineering solutions to the biotech and pharmaceutical industries in order to improve the success and economics of drug development. Its first product platform (HepatoPaCTM) is a micro-liver platform for advanced toxicity screening and drug discovery.
Primary Responsibilities / Reporting Requirements:
Implements and runs cell-based assays to characterize and validate liver culture models for screening drug metabolism and toxicity in collaboration with industrial partners. Analyzes data from experiments using statistical software packages. Prepares data summaries for dissemination to the group. Participates in technology development in liver tissue engineering. Exercises judgment within defined procedures to determine appropriate experimental action, and works on problems of moderate scope where analysis of data requires a review of identifiable factors. Works to further refine protocols for cell culture and downstream assays. Reports directly to Director of Research.
Degree Requirement / Prior Experience:
Bachelors or higher degree in a scientific or engineering discipline with 1-5 years prior experience working in a laboratory, preferably in an industrial setting. Mammalian cell culture experience, especially with primary liver cells (i.e. hepatocytes), is highly desirable. Basic knowledge of cell-based immunoassays (ELISAs, Western blots, colorimetric assays), PCR and aseptic technique using bio-safety cabinets is required.
Key Personality Traits:
Goal-oriented, highly motivated, strong work ethic, can solve problems with a hands-on approach, willing to learn new concepts/ techniques, can communicate research effectively in an interdisciplinary setting, and comfortable working in a start-up environment with an entrepreneurial mindset.
Compensation:
Hepregen offers competitive pay, a high growth environment, and a benefits package that includes stock options as well as participation in health and dental plans; 401(k) retirement plan; life insurance; and disability coverage.
Please send cover-letter and resume to: careers@hepregen.com
Hepregen Corporation located at 200 Boston Avenue, Medford MA 02155 is an equal opportunity employer. All candidates must be legally available to work in the United States.
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