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Must have at least a B.Sc. degree in biological sciences, preferably in biochemistry with a good understanding of protein and DNA. Previous work experience in a research laboratory is highly preferred. The position requires some molecular biology techniques such as basic PCR, cloning, plasmid DNA preparation; growing bacteria culture and maintaining insect cell culture for protein expression; and protein purification using affinity resins and column chromatography. Tissue culture experience is a valuable asset.
EMD Serono, Inc., an affiliate of Merck KGaA, Darmstadt, Germany, is a leader in the US biopharmaceutical arena, integrating cutting-edge science with unparalleled patient support systems to improve people’s lives. The company has strong market positions in neurodegenerative diseases, MS and endocrinology. EMD Serono is also a leader in fertility treatments. With a clear focus on the patient and a leadership presence in the biopharmaceutical industry, EMD Serono’s US footprint continues to grow, with more than 1000 employees around the country and fully integrated commercial, clinical and research operations in the company’s home state of Massachusetts.
Description
EMD Serono is currently looking for a motivated and talented scientist to expand the Neurodegenerative Diseases group in Cambridge, MA.
The purpose of the position is to conduct experiments under routine supervision using disease-relevant in vitro models to support drug discovery project teams for the treatment of Parkinson’s disease.
*It is anticipated that this position will move to Billerica in 2011.
KEY TASKS & RESPONSIBILITIES
This individual will be an integral part of a team that will use molecular, genetic, imaging and biochemical techniques to investigate the molecular mechanisms of nervous system disorders and to participate in drug discovery projects. A background in neuroscience or neurodegenerative models related to Parkinson’s disease is desirable.
The incumbent is expected to contribute to studies for multiple projects and to present his/her research internally in group settings. The candidate must have the ability to work collaboratively with others and independently perform and analyze in vitro experiments in line with assignments from his/her supervisor.
Specific responsibilities include:
Generates and characterizes stable cell lines for the development of disease-relevant cell models
Prepares and maintains primary neuronal cultures (including dissection)
Develops cell-free and cell-based assays for the identification of lead molecules and for mechanism of action studies
Performs data analysis, interpretation of results and trouble-shooting
Prepares written documents for internal reports
Gives data presentation to research teams
Expected to be familiar with technical and scientific literature pertaining to assigned project
Supports general laboratory maintenance and safety
Qualifications
EDUCATION / LANGUAGES
B.S./B.A. in biological sciences with 2 - 5+ years related industry/research experience or M.S. with 2+ years related industry/research experience. Degree with an emphasis on neurobiology is preferred.
Verbal and written fluency in English is required
PROFESSIONAL SKILLS & EXPERIENCE
Proficiency in cell culturing and aseptic techniques
Experience in working with DNA and RNA (e.g. cloning, purification, PCR)
Experience with protein expression and analysis (e.g. cell transfection, Western blot, immunocytochemistry, subcellular fractionation)
Familiarity with multiple screening platforms (e.g. ELISA, fluorescence/luciferase-based assays) and use of plate readers
Pharmacology experience with evaluating drug effects in vitro
High level of computer literacy (e.g. Excel, Vector NTI, Prism)
PERSONAL SKILLS & COMPETENCIES
Highly self-motivated, organized and detail-oriented
High level of flexibility and adaptability to a changing environment
Excellent oral and written communication skills.
Must be able to work in a team
Click the link below and open the door to the future by submitting your resume today!
Dana-Farber Cancer Institute is looking for a Research Technician to join their team!
Candidates should have a firm understanding or prior experience with molecular biology techniques, including PCR, genotyping, cloning and tissue culture. Be able to work with rodents and organize large mouse breeding colonies.
Job Qualifications
BA, BS or higher degree.
Schedule
Full-time, 40 hours
If interested please visit our website at: www.dana-farber.org and apply to req number 19572
Brigham and Womens Hospital (BWH) is internationally known as a leader in cutting-edge research and technology and its commitment to patient-centered care. BWH has a rich legacy of excellence and has been consistently recognized as one of Americas best hospitals by US News and World Report.
GENERAL SUMMARY:
An animal research technician position is available in the laboratories of Benjamin Ebert, MD, PhD and Sandra McAllister, Ph.D. at Brigham and Women’s Hospital and members of Harvard Stem Cell Institute and the Broad Institute and Dana Farber/Harvard Cancer Center. Our laboratory uses genomics and a range of molecular and cellular biological techniques to elucidate the molecular basis of cancer and hematologic disorders and to develop and test novel therapeutics. We are looking for enthusiastic, self-motivated individuals who thrive in a fast-paced environment and can interact well with others as part of a team.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
1. Perform basic and advanced animal work including: maintain breeding colonies, injections, surgery, aseptic technique, bone marrow transplantation, tumor measurements, drug delivery, tissue harvesting and processing.
2. Perform experiments, which may be moderate to complex with increasing independence.
3. General knowledge of experimental design
4. Record and enter data into commonly used data analysis programs.
5. Familiarity and compliance with animal vivarium policies and procedures.
6. Maintain and perform experiments with cells in tissue culture, genomic DNA preparation, PCR reactions, gel electrophoresis, and reagent preparation.
7. Help to prepare presentations and written articles for publication.
8. Prepare laboratory reagents, chemicals, instruments, and maintain equipment.
9. Maintain supplies inventory in accordance with departmental procedures.
10. All other duties, as assigned.
QUALIFICATIONS:
Experience with general animal handling and surgical techniques are required. BA/BS with significant biological/physical science coursework are highly recommended. . Experience with techniques in molecular biology, cell biology, or computational biology is desirable.
Two years experience required.
SKILLS/ABILITIES/COMPETENCIES REQUIRED:
Must be able to logically and effectively structure tasks and set priorities.
Computer literacy.
Sound analytical and organizational skills.
Good oral and written communication skills.
Ability to identify potential problems and trouble-shoot solutions.
Demonstrated ability to analyze data and to think critically about experimental design.
SUPERVISORY RESPONSIBILITIES:
May be responsible for training and orienting new staff in animal husbandry and biochemical/molecular techniques.
WORKING CONDITIONS:
Laboratory environment: works with animal colonies, and occasionally works with radioactive isotopes, toxic chemicals, biohazards, and substances emitting unpleasant odors.
The successful candidate will perform biological and biochemical experiments aimed at the study of dengue virus biology and characterization of candidate anti-dengue virus inhibitors. This includes in vitro tissue culture experiments as well as experiments performed in murine models of virus infection/replication and pathogenesis. Laboratory techniques will include plasmid preps, PCR, real-time PCR (QPCR) and real-time RT-PCR (qRT-PCR), cloning; Southern, Northern, Western blot; immunoprecipitation; immunofluorescent staining, fluorescence microscopy; cell culture, and viral plaque assays. Initially, the candidate will be responsible for assisting current lab members with their projects and will also be responsible for helping with general lab tasks and maintenance. As the successful candidate demonstrates proficiency and efficiency in the lab, opportunities to work more independently will be available. The successful candidate will also perform related duties as required. A willingness to learn and a keen interest in experimental science are essential. This is a one-year grant funded position.
Basic Qualifications: College background or equivalent work experience in experimental science; 1-2 years related work experience (relevant coursework may count towards experience).
Additional Qualifications: Undergraduate degree in biology or 2 or more years in an academic research setting; prior experience in a research laboratory; comfort working with and around BL2 viruses, including dengue virus, hepatitis B, hepatitis C, poliovirus; prior experience with standard molecular biology and cell biology is preferred; tissue culture experience, virology experience and experience working with rodent models is preferred, but a willingness to learn these techniques is an acceptable substitute.
Pre-Employment Screening: Identity
All applicants must apply via our website (please see requistion number 20468BR):
An Associate Scientist (Level II) will work in the Research Biology Department at the ALS-Therapy Development Institute in Cambridge, MA to support in vivo and in vitro validation of candidate therapeutics in murine models of Amyotrophic lateral sclerosis (ALS). This candidate will be responsible for the following three jobs:
1. Weekly genotyping of transgenic mice using an in-house, automated Q-PCR platform.
2. Support and development of enzyme linked immunosorbent assays (ELISA) for weekly, large-scale pharmacokinetic studies.
3. Development of assays for protein characterization such as immunoblots, immunoprecipitation, and immunohistochemistry.
The candidate will work closely with internal in vitro and in vivo research teams, as well as external vendors, to support ongoing preclinical studies in a mouse model of ALS.
Academic Qualifications and Technical skills:
The candidate must have a BS or MS in biology, biochemistry, or molecular biology and 3+ years of experience in molecular biology techniques including nucleic acid purification, PCR, Q-PCR, ELISA, and immunoblotting. Working knowledge of spreadsheet programs, is also required. Ideally, the candidate will have experience with molecular biology automation.
Strong communication skills and a team orientation are essential.
The Amyotrophic Lateral Sclerosis Therapy Development Institute (ALS-TDI) is a non profit biotech focused on the discovery and development of therapeutics to treat ALS. The institute is a world leader in the pharmacological testing of lead candidates in animal models of ALS and has established expertise in the utilization of genomics and proteomics technologies for drug discovery.
Hepregen Corporation (200 Boston Ave, Suite 1500, Medford, MA 02155, www.hepregen.com) is a venture-backed startup out of the Massachusetts Institute of Technology focused on commercializing a portfolio of liver tissue engineering technologies. Hepregens mission is to provide innovative bioengineering solutions to the biotech and pharmaceutical industries in order to improve the success and economics of drug development. Its first product platform (HepatoPaCTM) is a micro-liver platform for advanced toxicity screening and drug discovery.
Implements and runs cell-based assays to characterize and validate liver culture models for screening drug metabolism and toxicity in collaboration with industrial partners. Analyzes data from experiments using statistical software packages. Prepares data summaries for dissemination to the group. Participates in technology development in liver tissue engineering. Exercises judgment within defined procedures to determine appropriate experimental action, and works on problems of moderate scope where analysis of data requires a review of identifiable factors. Works to further refine protocols for cell culture and downstream assays. Reports directly to Director of Research.
Degree Requirement / Prior Experience:
Bachelors or higher degree in a scientific or engineering discipline with 1-5 years prior experience working in a laboratory, preferably in an industrial setting. Mammalian cell culture experience, especially with primary liver cells (i.e. hepatocytes), is highly desirable. Basic knowledge of cell-based immunoassays (ELISAs, Western blots, colorimetric assays), PCR and aseptic technique using bio-safety cabinets is required.
Key Personality Traits:
Goal-oriented, highly motivated, strong work ethic, can solve problems with a hands-on approach, willing to learn new concepts/ techniques, can communicate research effectively in an interdisciplinary setting, and comfortable working in a start-up environment with an entrepreneurial mindset.
Compensation:
Hepregen offers competitive pay, a high growth environment, and a benefits package that includes stock options as well as participation in health and dental plans; 401(k) retirement plan; life insurance; and disability coverage.
Please send cover-letter and resume to: careers@hepregen.com
Hepregen Corporation located at 200 Boston Avenue, Medford MA 02155 is an equal opportunity employer. All candidates must be legally available to work in the United States.
We are seeking a motivated, experienced molecular biology research technician to join our next generation DNA sequencing team at Massachusetts General Hospital in Boston, MA. This individual will be responsible for the day to day operation of this core lab, including customer interactions, sequencing library construction, ordering supplies, and operating the Illumina GA IIx sequencing instrument. The successful candidate will be able to operating very independently, manage many projects at once, keep accurate records, and execute complex molecular biology protocols flawlessly.
The successful applicant will have excellent written and communication skills, be highly organized and rigorous, and have experience in modern molecular biology techniques (PCR, restriction enzymes, ligations, cloning). A bachelors/masters degree in biology or a related field is preferred. This is a rare opportunity to work closely with leaders in genetics, medicine, and molecular biology in a top research institution.
This is a two year position with the possibilities of extending the position.
If interested, please apply online at www.mghcareers.org and search for this specific job opening using Job ID # 2198216
The Clinical Laboratory Technologist will perform complex molecular-based clinical diagnostic assays and other tasks according to established operational procedures, within our CLIA-certified commercial genotyping laboratory.
MAJOR RESPONSIBILITIES AND DUTIES:
Purify and analyze DNA samples, using molecular genetics techniques that include PCR.
Routinely use liquid handling robotics and analysis equipment to perform genetic assays.
Analyze and interpret results which will be used to prepare test reports.
Work with supervisor to review and troubleshoot procedures.
Maintain complete documentation for data and results tracking, using our LIMS (Laboratory Information Management System).
Adherence to CLIA regulations: equipment maintenance, chemical inventory, sample tracking and SOP review.
Other related duties as assigned.
SUPERVISION:
Reports to Commercial Laboratory Manager. Must be able to work independently with strong attention to detail and results.
QUALIFICATIONS:
Bachelors degree in related scientific area required; 2-4 years of molecular biology experience preferred.
Familiarity with concepts, practices, and procedures related to genetic testing and genotyping.
Strong attention to detail; precision required.
CLIA or GLP laboratory experience preferred.
Prior computer experience required.
Ability to work well in a team environment required.
