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Biologist Postdoc Researcher (berkeley)
By | July 14, 2009
In the fight against global warming, the need to develop clean, green, and renewable sources of energy has become an international call to action. The Joint BioEnergy Institute (JBEI), a newly established U.S. Department of Energy (DOE) Bioenergy Research Center, is responding by assembling an outstanding team of bioenergy scientists and researchers to meet the challenge. To learn more about JBEI, visit www.jbei.org.
JBEI is a multi-organizational research center that comprises world-class resources and expertise in one facility, which is located in Emeryville, California. The goal of JBEI is to use rapidly advancing scientific techniques, such as systems and synthetic biology, to accelerate the development of the nations biofuels industry. JBEI is managed by Lawrence Berkeley National Laboratory with partner employees from five internationally recognized scientific institutions: Sandia National Laboratories, Lawrence Livermore National Laboratory, the University of California at Davis, the University of California at Berkeley, and the Carnegie Institution for Science.
Researchers at JBEI have been brought together for one purpose: to break the barriers so that lignocellulosic material can be efficiently converted into biofuels. To help meet this goal, JBEI emphasizes integration and teamwork to develop engineering plants for better processing, efficient protocols for pretreatment, and optimized microorganisms for increased biofuels production. Our start-up team consists of staff scientists, postdoctoral researchers, and technicians in the areas of plant biology, enzymology, biochemical and chemical engineering, functional genomics, applied microbiology, fermentation, molecular biology, genetics, and bioinformatics. If you possess research expertise in any of these areas, we invite you to consider joining our world-class team.
The Fuels Synthesis Division of the Joint BioEnergy Institute (JBEI) has an opening for a postdoctoral researcher with experience in anaerobic and molecular microbiology to engage in research on novel pathways of bacterial biosynthesis of fuel constituents. The successful candidate will participate in research to identify genes associated with biosynthesis of specific fuel molecules from anaerobic bacteria using whole-genome cDNA microarrays (including bioinformatic analysis of the results), heterologous expression of candidate genes in E. coli and assays of relevant activity, and other approaches.
What you will get to do:
Optimize cultivation of target anaerobic bacteria (liquid cultures).
Conduct gene expression experiments on target bacteria examining exposure/growth conditions with varying fuel biosynthesis fluxes.
Assist in the identification of putative fuel biosynthesis genes based on genomic and transcriptomic data, and express the target genes in E. coli to assess relevant metabolic activity.
Participate in the preparation of manuscripts for publication and in the development of funding proposals and patents.
Participate in group meetings and seminars.
Perform research lab procedures efficiently and with attention to necessary quality control procedures.
Maintain well-documented laboratory notebook (e-notebooks will be used).
Support in vitro assays by performing analyses by gas chromatography/mass spectrometry.
Support experiments by purifying tagged proteins and running SDS-PAGE gels and Western blots.
What you need to succeed:
Recent Ph.D. in Microbiology, Biochemistry, Molecular and Cell Biology, or a related field of science.
Extensive experience in the cultivation of anaerobic bacteria and use of an anaerobic glove box.
Extensive experience performing laboratory research using standard techniques of molecular biology, including extraction and manipulation of RNA and genomic and plasmid DNA, gel electrophoresis, PCR (Including qPCR and RT-qPCR and primer design), and cloning.
Familiarity with common bioinformatics tasks (e.g., BLAST searches).
Ability to work with guidance from a director or principal investigator and collaborators and well as to conduct experiments independently, maintain appropriate records, and manage data.
Excellent written communication skills along with good organizational and interpersonal skills.
Ability to work in a team environment and effectively interact with a broad range of colleagues.
Experience with cDNA microarrays.
Experience with gas chromatography/mass spectrometry or other chromatographic techniques.
Experience with standard protein biochemistry techniques (such as SDS-PAGE, Western blots).
In addition to the intellectual and cultural advantages of being a member of the LBNL community, we offer employees outstanding health and welfare benefits, and programs for a secure retirement that are considered to be among the finest.
Learn more about this opportunity and apply at http://jobs.lbl.gov/LBNLCareers/details.asp?jid=22906&p=1sid=2027.
Berkeley Lab is an Affirmative Action/Equal Opportunity Employer committed to the development of a diverse workforce.
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